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【目的】探讨抗癌防移片抑制4T1乳腺癌血管生成的机理。【方法】选用BALB/c小鼠建立4T1乳腺癌模型,随机分成空白对照组、模型组、环磷酰胺(CTX,剂量为0.04 g·kg-1·d-1)组和抗癌防移片组(剂量为5.2 g·kg-1·d-1),分别给予药物或生理盐水,给药4周后处死小鼠,测量剥离瘤质量,计算剥离瘤质量抑制率以及计数肺转移结节数,并通过免疫组织化学染色法测定肿瘤微血管数与血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)表达。【结果】与模型组比较,抗癌防移片组剥离瘤质量显著减轻(P<0.05),肺转移结节数目显著减少(P<0.05),且剥离瘤微血管数与VEGF表达有降低趋势。【结论】抗癌防移片可能通过下调VEGF、抑制肿瘤微血管生成从而抑制4T1小鼠乳腺癌生长与转移。
【Objective】 To investigate the mechanism of anti-cancer anti-metastasis inhibiting 4T1 breast cancer angiogenesis. 【Methods】 BALB / c mice were used to establish 4T1 breast cancer model and randomly divided into blank control group, model group, cyclophosphamide (CTX, 0.04 g · kg-1 · d-1) The rats were sacrificed 4 weeks after the administration, the quality of the stripping tumor was measured, the rate of stripping tumor mass was calculated, and the number of lung metastasis nodules , And the number of microvessel and the expression of vascular endothelial growth factor (VEGF) were measured by immunohistochemical staining. 【Results】 Compared with the model group, the quality of the stripping tumor in anti-cancer anti-platelet group was significantly reduced (P <0.05), and the number of metastatic lung nodules was significantly decreased (P <0.05), and the microvessel number and VEGF expression in stripping tumor decreased. 【Conclusion】 Anti-cancer anti-metastasis may inhibit the growth and metastasis of breast cancer in 4T1 mice by down-regulating VEGF and inhibiting tumor angiogenesis.