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包虫病在肯尼亚是一种重要的寄生虫病,特别是在Furkana地区,为世界上人体包虫病发病率最高的地方。该病除人感染以外,家畜、野生动物感染均有报告。为了寻求一种理想的抗原,采用耐热的细粒棘球蚴液(HCF)抗原—“880”抗原,观察其在肯尼亚人体包虫病的诊断和血清流行病学调查中的作用。抗原制备:按不同种别在各屠宰场收集HCF,加0.1%叠氮钠,于-20℃中冰冻保存。融化后经离心、过滤、浓缩,并以10ml分装,置高压蒸煮器内加温至110℃10分钟
Echinococcosis is an important parasitic disease in Kenya, especially in the area of Furkana, which has the highest incidence of human echinococcosis in the world. The disease in addition to human infection, domestic animals, wildlife infections are reported. In order to find an ideal antigen, we used the thermophilic antigen of hydatid cyst fluid (HCF) - “880” to investigate its role in the diagnosis and serological epidemiology of human echinococcosis in Kenya. Preparation of Antigen: HCF was collected at different slaughterhouses by adding 0.1% sodium azide and stored frozen at -20 ° C. After thawing, centrifugation, filtration, concentration, and 10ml sub-installed high-pressure cooker was warmed to 110 ℃ 10 minutes