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目的探讨生存素在人正常胃黏膜细胞株及胃癌细胞株中的表达和白细胞介素(IL)-1β对胃黏膜细胞生存素表达的影响。方法用RT-PCR和Westernblot法检测人正常胃黏膜细胞株(GES-1)及3株胃癌细胞(AGS、SGC-7901、BCG-823)和经IL-1β处理后的GES-1、AGS细胞中生存素的表达。结果 3株胃癌细胞均有生存素表达,正常胃黏膜细胞无生存素表达。IL-1β不刺激正常胃黏膜细胞生存素的表达,可剂量依赖性增加胃癌细胞生存素的表达。结论胃癌细胞均有生存素表达,正常胃黏膜细胞不表达生存素;IL-1β对胃癌细胞生存素的表达有正向作用,表明IL-1β在胃癌的发展过程中有一定作用。
Objective To investigate the expression of survivin in human normal gastric mucosa cell line and gastric cancer cell line and the effect of interleukin (IL) -1β on gastric mucosal cell survivin expression. Methods GES-1 and 3 gastric cancer cells (AGS, SGC-7901 and BCG-823) and GES-1 and AGS cells treated with IL-1β were detected by RT-PCR and Western blotting. In the expression of survivin. Results All three gastric cancer cells had survivin expression and no survivin expression in normal gastric mucosa. IL-1|Â did not stimulate the expression of survivin in normal gastric mucosa, and increased the expression of survivin in gastric cancer cells in a dose-dependent manner. Conclusions Survivin is expressed in gastric cancer cells and survivin is not expressed in normal gastric mucosa cells. IL-1β has a positive effect on the expression of survivin in gastric cancer cells, indicating that IL-1β plays a role in the development of gastric cancer.