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通过模拟试验研究了正常水分和淹水条件下氢醌影响的土壤脲酶动力学特征。结果显示,培养时间、土壤类型、土壤水分及其相互作用显著影响土壤脲酶动力学参数的变化。与对照相比,HQ使土壤脲酶Km增加和Vmax降低,表明HQ属于典型的混合型抑制剂。随着培养时间延长,在正常水分和淹水条件下,HQ处理土壤脲酶Km降低,而Vmax和Vmax/Km升高。褐土脲酶Km的增加幅度及Vmax和Vmax/Km的降低幅度远远大于其它3种供试土壤。与正常水分相比,淹水对土壤脲酶动力学参数无显著性影响。相关分析表明,土壤脲酶动力学参数Km、Vmax和Vmax/Km与土壤理化性质存在显著的相关关系。
The kinetic characteristics of soil urease affected by hydroquinone under normal and submerged conditions were studied by means of simulation experiments. The results showed that incubation time, soil type, soil moisture and their interactions significantly affected the changes of soil urease kinetic parameters. Compared with the control, HQ increased soil urease Km and decreased Vmax, indicating that HQ is a typical mixed inhibitor. With the prolongation of culture time, the soil urease Km decreased and the Vmax and Vmax / Km increased under the condition of normal water and flooding. The increase of brown soil urease Km and the decrease of Vmax and Vmax / Km were far greater than the other three kinds of soil tested. Compared with normal water, flooding had no significant effect on soil urease kinetic parameters. Correlation analysis showed that soil urease kinetic parameters Km, Vmax and Vmax / Km had significant correlation with soil physical and chemical properties.