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决明子是一种传统中药,具有降低血清胆固醇的作用.通过凝胶排阻及离子交换层析技术从决明子中分离出一种具有抑制胆固醇合成作用的新蛋白PeakⅡ-3,将之命名为新决明子蛋白.质谱分析表明该蛋白分子量为19.7kD,等电聚焦(IEF)电泳结果显示其等电点为4.80.SDS-PAGE及等电聚焦电泳的结果均为单一条带,表明新蛋白为均一组分.对该蛋白N-端进行测序,结果为IPYISASFPLNIEFPSE,用BLAST在NCBI-BLASTP库中搜索没有找到同源蛋白.圆二色谱结果显示该蛋白具有12.5%的α螺旋,55.6%的β折叠,31.9%的无规卷曲.
Cassia seed is a traditional Chinese medicine with the function of reducing serum cholesterol.A new protein named Peak II-3 with the function of inhibiting cholesterol synthesis was isolated from cassia seed by gel exclusion and ion-exchange chromatography and named as new cassia Mass spectrometry analysis showed that the molecular weight of the protein was 19.7kD, isoelectric focusing (IEF) electrophoresis showed that the isoelectric point of 4.80.SDS-PAGE and isoelectric focusing electrophoresis results are single band, indicating that the new protein is uniform group The N-terminal of this protein was sequenced and the result was IPYISASFPLNIEFPSE, no homologous protein was found by BLAST search in NCBI-BLASTP library.The circular dichroism showed 12.5% α-helix, 55.6% β-sheet, 31.9% random curl.