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采用CCl4致小鼠急、慢性肝损伤模型,平行动态观察了肝组织学改变与同期血清脯肽酶(PLD)和丙氨酸转氨酶(ALT)变化关系。急性肝损伤中,血清PLD和ALT活性均明显升高,且二者变化呈显著正相关(n=100,r=0.3853.P<0.01)。PLD峰值期为注射CCl4后16h,与肝坏死最严重的时相一致。PLD峰值和恢复时间均早于ALT。肝硬化形成大致可分为胶原纤维增生(1~6周),肝硬化形成(6~9周)和肝硬化(9~12周)3个阶段。其间血清PLD活性第1周开始升高、3~6周达高峰,而后开始下降,9周后降至正常,而ALT无明显变化。结果表明,血清PLD活性测定是一项诊断急性肝损伤的敏感指标,除外急性肝损伤、PLD活性在某种程度能较好地反映进展性肝纤维化。
The acute and chronic liver injury model induced by CCl4 in mice was used to dynamically observe the changes of liver histology and the changes of serum procalcitonin (PLD) and alanine aminotransferase (ALT) in parallel. Serum levels of PLD and ALT were significantly increased in acute liver injury, and there was a significant positive correlation between them (n = 100, r = 0.3853, P <0.01). The peak PLD was 16 h after injection of CCl 4, consistent with the most severe phase of hepatic necrosis. Both PLD peak and recovery time were earlier than ALT. The formation of cirrhosis can be divided into three stages: hyperplasia of collagen fibers (1-6 weeks), cirrhosis (6-9 weeks) and cirrhosis (9-12 weeks). Serum PLD activity increased during the first week, peaked at 3-6 weeks, then began to decline, and then dropped to normal after 9 weeks, while ALT had no significant change. The results showed that the determination of serum PLD activity is a sensitive indicator of acute liver injury diagnosis, except for acute liver injury, PLD activity to a certain extent, can better reflect the progress of liver fibrosis.