利用RNA干涉技术构建水稻DDB1(Damaged DNA binding protein 1)基因不同启动子驱动植物表达载体,DDB1-RNAi和DDB1-glu-RNAi.通过根癌农杆菌介导转入水稻愈伤,经组织培养成功获得转基因植株.半定量RT-PCR分析显示,与野生型植株相比,两组转基因植株幼叶内DDB1的表达量均有不同程度的降低.花粉染色结果显示,转基因植株花粉染色异常,败育率高;植株表现出明显的雄性半不育;秕谷率均有不同程度的提高;并且以上表型在DDB1-RNAi转基因植株中均比在DDB1-glu-RNAi转基因植株中明显.因此,推断DDB1基因与水稻的生殖发育有着密切的关系. DDB1-RNAi and DDB1-glu-RNAi driven by different promoters of DDB1 (Damaged DNA binding protein 1) gene were constructed by RNA interference technique and transformed into rice callus by Agrobacterium tumefaciens. The tissue culture was successful The transgenic plants were obtained by semi-quantitative RT-PCR analysis.The DDB1 expression in young leaves of the two transgenic plants was reduced to some extent compared with the wild-type plants.The results of pollen staining showed that the transgenic plants were pollen-stained abnormally and aborted High rate; the plants showed obvious male semi-sterile; the rate of stoloniferous valley increased in varying degrees; and the above phenotype was more obvious in DDB1-RNAi transgenic plants than in DDB1-glu-RNAi transgenic plants. The DDB1 gene is closely related to the reproductive development of rice.