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研究不同根序细根根际土壤微生物群落组成结构对深入了解根系-微生物互作关系具有重要意义.本研究采用Illumina Mi Seq测序平台,对杨树人工林非根际土壤和不同根序细根根际土壤的真菌群落结构进行分析.物种注释结果显示:杨树1~2级根(R1)、3级根(R2)和4~5级(R3)根际及非根际土壤(NR)中分别包含128、124、130和101个真菌属,表明杨树根际存在对真菌群落构建的选择性机制.不同根序根际土壤中相对丰度>1%的真菌属有7个,木霉属在1~2级根根际土壤中丰度较高,毛孢子菌属和曲霉属分别是3级根和4~5级根根际土壤中丰度最高的真菌属.α多样性指数表明:根际土壤真菌的多样性在不同根序间存在显著差异,低级根显著高于高级根(P<0.05).β多样性指数表明:真菌群落随着序级的升高差异性不断上升,相似性不断降低.不同根序细根根际真菌群落的趋异化组成和结构与细根功能具有密切关系.
Studying rhizosphere soil microbial community composition structure with different rhizomatous roots is of great importance to understand the interaction between root and soil microorganisms.In this study, Illumina Mi Seq sequencing platform was used to analyze the relationships between non-rhizosphere soil and root The rhizosphere soil fungal community structure was analyzed. Species annotation results showed that rhizosphere and non-rhizosphere soil (NR) of 1st to 2nd roots (R1), 3rd roots (R2) and 4th to 5th grades (R3) Including 128, 124, 130 and 101 fungi, respectively, suggesting that there is a selective mechanism for the establishment of fungal communities in the rhizosphere of poplar.Among the rhizosphere soils of different rhizosphere, there are 7 fungi with relative abundance> 1%, Trichoderma The root abundance of rhizosphere soil was higher in the first to the second grade rhizosphere soils, while the genus Coccidioides and Aspergillus were the most abundant fungi in the rhizosphere soils of Grade 3 and 4 to 5. The diversity index of α showed that: The diversity of rhizosphere soil fungi showed significant differences among different root orders, and the lower roots were significantly higher than that of the higher roots (P <0.05) .β diversity index indicated that the diversity of fungal communities increased with the increase of order Sex decreased continuously.Differentiation composition and structure of fungi in rhizosphere fungi of different root orders with fine root function close relationship.