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用亲和层析法提纯马抗ＡＦＰ，采用氯胺Ｔ改良法标记１２５Ｉ，经ＳｅｐｈａｄｅｘＧ５０柱分离，以ｒ计数与紫外同步监测进行标记物的收集。并对１２５Ｉ－抗ＡＦＰ制备方法及其稳定性等进行探讨和测定。结果：１２５Ｉ和抗ＡＦＰ的利用率分别为６０％～８０％和５０％～７３％；标记率为６５％～８３％，１２５Ｉ－抗ＡＦＰ的比放射性为５５．５～７４ＭＢｑ／ｍｇ；放化纯度５ｄ内为９７％～９８％，１４ｄ为９３％；抗体活性标记后与标记前基本不变，４℃保存５ｄ和１４ｄ均无降低。表明１２５Ｉ－抗ＡＦＰ的生物活性及稳定性良好。
Affinity chromatography was used to purify the horse anti-AFP, using the chloramine T modified method labeled 125I, separated by SephadexG50 column, with r count and UV synchronization monitoring for the collection of the marker. The preparation and stability of 125I-anti-AFP were also discussed and measured. Results: The utilization rates of 125I and anti-AFP were 60% to 80% and 50% to 73%, respectively; the labeling rate was 65% to 83%; the specific radioactivity of 125I-anti-AFP was 55.5 to 74 MBq/mg; The purity was 97%-98% within 5 days and 93% after 14 days. The activity of the antibody remained unchanged after labeling, but it was not decreased after 5 days and 14 days of storage at 4°C. It shows that the biological activity and stability of 125I-anti-AFP are good.