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目的:观察复方雷公藤颗粒对再生障碍性贫血(简称再障)小鼠的疗效及对骨髓CD4+,CD8+,CD4+/CD8+T淋巴细胞表达的影响。方法:建立再障小鼠模型,分为5组,正常组及模型组小鼠(给予同体积的生理盐水ig),复方雷公藤低、高剂量组(分别为生药10.63,13.29 g.kg-1,ig)、十一酸睾酮(22 mg.kg-1,ig),连续给药35 d,取骨髓单个核细胞培养24 h后,流式细胞术检测CD4+,CD8+,CD4+/CD8+T淋巴细胞的表达水平。结果:模型组CD4+,CD8+,CD4+/CD8+各指标均较正常组下降,其中CD4+,CD4+/CD8+T淋巴细胞明显低于正常组(P<0.05);十一酸睾酮组与模型组相比,CD4+,CD8+均有所下降,但CD4+/CD8+T细胞较之上升,但统计学均无明显差异,复方雷公藤颗粒低、高剂量组与模型组相比,CD4+,CD8+,CD4+/CD8+均升高,其中CD4+,CD4+/CD8+升高有统计学意义(P<0.05),与十一酸睾酮组相比差异有统计学意义(P<0.05)。结论:复方雷公藤颗粒能调节骨髓T细胞的异常状态,恢复T细胞及其亚群之间的平衡,促进骨髓造血功能的恢复。
Objective: To observe the curative effect of Compound Tripterygium Wilfordii Granule on the mice with aplastic anemia (Aplastic Anemia) and its effect on the expression of CD4 +, CD8 + and CD4 + / CD8 + T lymphocytes in bone marrow. Methods: Aplastic anemia mice models were established and divided into 5 groups: normal group and model group (given the same volume of normal saline ig), compound Tripterygium low and high dose groups (10.63, 13.29 g.kg- 1, ig) and testosterone undecanoate (22 mg.kg-1, ig) for 35 days. The bone marrow mononuclear cells were cultured for 24 h. The levels of CD4 +, CD8 +, CD4 + / CD8 + T Lymphocyte expression levels. Results: The levels of CD4 +, CD8 + and CD4 + / CD8 + in model group were significantly lower than those in normal group (P <0.05). The levels of CD4 + and CD4 + / CD8 + T lymphocytes in model group were significantly lower than those in normal group CD4 +, CD8 + and CD4 + / CD8 + T cells were lower than those in the untreated group, but CD4 + / CD8 + T cells were higher than those in the untreated group (P <0.05). The difference was statistically significant (P <0.05) compared with testosterone undecanoate group. Conclusion: Tripterygium Wilfordii Granule can regulate the abnormal status of bone marrow T cells, restore the balance between T cells and their subsets, and promote the recovery of bone marrow hematopoietic function.