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为明确尖孢镰刀菌Fusarium oxysporum不同专化型菌株的遗传多样性,利用MIDI微生物脂肪酸鉴定系统分析了来源于5个专化型的27株菌株的磷脂脂肪酸种类和含量,并进行了磷脂脂肪酸聚类分析。结果表明,供试尖孢镰刀菌中共检测到9种脂肪酸,在兰氏距离为25.98时分为3个类群,即主要脂肪酸、微量脂肪酸和偶现脂肪酸,其中主要脂肪酸有16:0、18:0、18:1cis9(ω9)和18:2cis9,12/18:0a,含量共占总磷脂脂肪酸的96.41%;微量脂肪酸有14:0、15:0、16:1cis9(ω7)和20:0,偶现脂肪酸仅有17:1(ω11)。不同专化型菌株的主要脂肪酸仅18:2cis9,12/18:0a存在显著差异,微量脂肪酸除15:0外其余3种均有显著差异。聚类分析结果表明,以供试的全部27株菌株聚类时,其磷脂脂肪酸与寄主专化型间无明显相关性,以寄主来源为同科(葫芦科或茄科)的菌株聚类时,其磷脂脂肪酸与寄主专化型间存在着一定的对应关系。
In order to clarify the genetic diversity of Fusarium oxysporum strains, the types and contents of phospholipid fatty acids in 27 strains of five specialized strains were analyzed by using MIDI microbial fatty acid identification system. The phospholipid fatty acid poly Class analysis The results showed that 9 kinds of fatty acids were detected in Fusarium oxysporum and divided into three groups at the same distance of 25.98, namely the main fatty acids, the trace fatty acids and the occasional fatty acids, the main fatty acids were 16: 0, 18: 0 , 18: 1cis9 (ω9) and 18: 2cis9, 12/18: 0a, accounting for 96.41% of the total phospholipid fatty acids; the trace fatty acids were 14: 0,15: 0,16: 1cis9 (ω7) Even fatty acids only 17: 1 (ω11). There were significant differences in the major fatty acids of the different special strains only 18: 2cis9, 12/18: 0a, the other three kinds of trace fatty acids except 15: 0 were significantly different. The results of cluster analysis showed that there was no significant correlation between phospholipid fatty acids and host-specific types when clustering all the 27 isolates tested. When the host strains were clustered by isolates from the same families (Cucurbitaceae or Solanaceae) , Its phospholipid fatty acids and host-specific type there is a certain correspondence between.