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目的:揭示125I粒子照射膀胱癌的生物学效应,为临床应用125I粒子治疗膀胱癌提供实验依据.方法:把T739近交系小鼠膀胱低分化移行细胞癌动物8只随机分为两组,实验组和对照组,每组4只.在小鼠瘤体内植入MSI-125型125I粒子,在对照组小鼠瘤体内相应部位植入粒子空壳,继续饲养24d后处死.在距粒子植入部位0.6,1.2,1.8cm的小鼠肿瘤内分别取3块组织,分离后行癌细胞培养,显微镜下动态观察细胞病理形态变化及生长、增殖状况.统计克隆形成率,计算克隆形成抑制率.结果:①实验组细胞数量明显少于对照组.实验组不同部位的培养细胞数量有较明显差异.②实验组克隆形成抑制率为41.84%.③两组不同部位所取组织细胞的总体克隆形成率有显著差异(P<0.01),实验组不同部位间亦有显著差异(P<0.01).结论:植入的125I粒子主要是靠γ射线的穿透力在有效射程内直接杀伤癌细胞,随着距离延长,辐射剂量减少,γ射线对癌细胞杀伤作用下降.
OBJECTIVE: To reveal the biological effects of 125I particles on bladder cancer and to provide experimental basis for the clinical application of 125I particles in the treatment of bladder cancer.Methods: 8 T739 inbred mouse bladder poorly differentiated transitional cell carcinomas were randomly divided into two groups, Group and control group with 4 mice in each group.The MSI-125 125I seeds were implanted in the tumor of mouse and the empty shell of the mice was implanted in the corresponding part of the tumor in the control group, The tumor tissues of 0.6, 1.2 and 1.8 cm in each site were taken from three tumor tissues, respectively. After separation, the cancer cells were cultured and the morphological changes, growth and proliferation of cells were observed dynamically under the microscope. The clonogenic rates were calculated. Results: ① The number of cells in the experimental group was significantly less than that of the control group.The numbers of cultured cells in different sites of the experimental group were significantly different.② The inhibition rate of cloning formation in the experimental group was 41.84% .③The total clonal formation of the tissue cells in two groups (P <0.01). There was also a significant difference between different sites in the experimental group (P <0.01) .Conclusion: The implanted 125I particles directly kill the cancer cells in the effective range by the penetrating power of γ rays, With the distance extension , Radiation dose reduction, decrease cancer killing gamma] ray pair.