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目的探讨nesfatin-1对内脏高敏感大鼠内脏敏感性和结肠动力的影响。方法将新生2 d龄雄性Spragne-Dawley大鼠随机分为空白对照组和模型组,制备内脏高敏感性大鼠模型。模型组分为干预对照组、低浓度干预组、中浓度干预组和高浓度干预组,分别给予侧脑室注射0.5μl 0.9%氯化钠、0.5μmol/L nesfatin-1、5μmol/L nesfatin-1、50μmol/L nesfatin-1,评定各组大鼠内脏敏感性,同时记录各组大鼠结肠电生理指标,对实验结果进行差异性统计分析。结果空白对照组和模型组大鼠体重差异无统计学意义(P>0.05),模型组大鼠较空白对照组易激惹。模型组大鼠内脏敏感性较空白对照组高(P<0.05),同时组织学排除器质性病理改变的存在,提示造模成功。三种不同浓度nesfatin-1干预组大鼠内脏敏感性明显高于干预对照组(P<0.05),且低、中和高浓度干预组之间大鼠内脏敏感性差异亦有统计学意义(P<0.05)。干预对照组大鼠快慢波频率、快慢波最大振幅、收缩波数和收缩波振幅指数均低于低、中和高浓度干预组;低、中浓度干预组快慢波频率和快慢波振幅与高浓度干预组比较,差异有统计学意义,但低浓度干预组与中浓度干预组间收缩波数和收缩波振幅指数差异无统计学意义。结论中枢途径应用nesfatin-1可以影响内脏高敏感性大鼠内脏敏感性和结肠动力。
Objective To investigate the effect of nesfatin-1 on visceral sensitivity and colonic motility in visceral hypersensitivity rats. Methods Male Sprague-Dawley rats of 2 days old were randomly divided into blank control group and model group to prepare visceral hypersensitivity rat model. The model group was divided into intervention group, low concentration intervention group, medium concentration intervention group and high concentration intervention group, and were given intracerebroventricular injection of 0.5μl 0.9% sodium chloride, 0.5μmol / L nesfatin-1,5μmol / L nesfatin-1 , 50 micromol / L nesfatin-1. The visceral sensitivity of rats in each group was evaluated. At the same time, the colonic electrophysiological indexes of rats in each group were recorded, and the statistical analysis was carried out on the experimental results. Results There was no significant difference in weight between the blank control group and the model group (P> 0.05). The model group was more irritable than the blank control group. The visceral sensitivity in the model group was higher than that in the blank control group (P <0.05), meanwhile histological examination excluded the existence of organic pathological changes, suggesting that the model was successful. The visceral sensitivity of three different concentrations of nesfatin-1 intervention group was significantly higher than that of the intervention control group (P <0.05), and the difference of visceral sensitivity between the intervention groups of low, middle and high concentration was also statistically significant (P <0.05). The frequency of fast and slow waves, the maximum amplitude of fast and slow waves, the amplitude of contractility wave and wave amplitude in intervention group were lower than those in low, middle and high concentration intervention group. The amplitude of fast and slow wave and the amplitude of fast and slow wave in high and low concentration group Group, the difference was statistically significant, but there was no significant difference between contraction wave number and contraction wave amplitude index between low-concentration intervention group and middle-concentration intervention group. Conclusion The central route of nesfatin-1 can affect visceral hypersensitivity in rat visceral sensitivity and colonic motility.