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目的研制专用于活体大鼠尺骨四点弯曲疲劳试验的夹具装置,制作活体微损伤动物模型,探讨活体大鼠疲劳试验方法并分析其骨生物力学性能。方法根据四点弯曲原理研制与PLD-5010型电子疲劳损伤试验机配套的夹具装置,该夹具由装载大鼠的载物盘和活体大鼠左(右)前肢加载装置2部分组成,后者包括上连接杆、弹簧上连接轴、线弹性弹簧、四点弯曲试验装置主体和下连接杆等零部件。选择4只12周龄雌性SD大鼠,随机分成对照组和负载组,每组各2只。2%戊巴比妥钠腹腔注射麻醉后,负载组大鼠用该夹具进行活体双侧尺骨四点弯曲疲劳损伤试验。负载频率:2 Hz,负载:10000次,负载力0.055 5 N/g(体重),隔天1次,共2次;对照组不行疲劳损伤试验。试验结束后处死2组大鼠,取双侧尺骨,用碱性品红染色,塑料包埋,磨片至50～80μm,Leica显微镜下发现骨微损伤后,用CCD头摄取图像并输入计算机储存。结果成功制作了疲劳夹具,可与骨电子疲劳损伤试验机配套应用。在负载组大鼠活体损伤的左右尺骨4个标本共20张磨片中均发现有微破裂,对照组未发现前述变化。结论该夹具适合于PLD-5010型电子疲劳损伤试验机,经过四点弯曲疲劳试验可初步建立活体大鼠尺骨微损伤模型,该活体大鼠疲劳试验方法可用于进一步的微损伤研究。
OBJECTIVE: To develop a clamp device specially designed for the four-point bending fatigue test of live rat ulnar, to make a living animal model of micro-damage, to explore the fatigue test of living rats and to analyze its biomechanical properties. Methods According to the four-point bending principle, a fixture device matched with the PLD-5010 electronic fatigue damage tester was developed. The fixture consisted of a rat-carrying tray and a live rat left (right) forelimb loading device 2 which consisted of On the connecting rod, the spring on the connecting shaft, linear spring, four-point bending test device body and the lower connecting rod and other components. Four 12-week-old female Sprague-Dawley rats were randomly divided into control group and loading group, 2 in each group. 2% pentobarbital sodium anesthesia after intraperitoneal injection, the load group of rats using the fixture for four-point bending of the bilateral ulna fatigue test. Load frequency: 2 Hz, load: 10000 times, load power 0.055 5 N / g (body weight), every other day, a total of 2 times; the control group no fatigue damage test. At the end of the experiment, two groups of rats were sacrificed. The ulnar ulnar bone was stained with basic magenta and embedded in plastic and polished to 50 ~ 80μm. After the lesion was detected by Leica microscope, the images were captured by CCD and input into computer . The results of the successful production of fatigue fixture, can be used with bone fatigue testing machine electronic matching applications. Micro-rupture was found in all 20 specimens of the left ulnar ulnar lesion in the injured group of rats in loading group, while no change was found in the control group. Conclusion The fixture is suitable for the PLD-5010 electronic fatigue damage testing machine. After four-point bending fatigue test, a micro-injury model of the ulnar ulna in vivo can be established initially. The fatigue test of the living rat can be used for further micro-damage study.