Patchy distribution of high As groundwater has normally been observed in As-affected areas. Spatial and temporal evolutions help in better understanding mechanisms of As mobilization and in developing effective strategies for ensuring drinking water safety. Four multilevel samplers were installed approximately along the groundwater flow path to investigate spatial and temporal variations in groundwater As in the Hetao basin, Inner Mongolia. Both water chemistry and groundwater level were monitored for about two years. Groundwater As concentration generally showed increasing trends, and Eh values showed decreasing trends along the flow path, indicating that As was mobilized via reductive dissolution of Fe oxides. However, in evaporation discharge area, shallow groundwater As was generally lower than those upstream and downstream. In addition to evaporation, siderite and pyrite precipitations controlled groundwater As concentrations. The negative correlations between As concentration and SIpyrite (or SIsiderite) implied that siderite and pyrite precipitations wold scavenge groundwater As and lower As concentration. Temporal variation showed different trends in different locations. It may reflect replenishment of high/low As groundwater for the increase/decrease in As concentrations, resulting from water level fluctuation. The increase trends in groundwater As concentrations at depth around 15m in the discharge areas would result from the increase in the recharge of underlying groundwater (20m) with high As concentration due to enhanced evaporation in the seasons with high water levels. The investigation suggested that monitoring of groundwater As should be routinely carried out to ensure the drinking water safety in the As-affected areas. Patchy distribution of high As groundwater has normally been observed in As-affected areas. Spatial and temporal evolutions help in better understanding mechanisms of As mobilization and in developing effective strategies for ensuring drinking water safety. Four multilevel samplers were installed as along the groundwater flow path To investigate spatial and temporal variations in groundwater As in the Hetao basin, Inner Mongolia. Both water chemistry and groundwater levels were monitored for about two years. Groundwater As concentration generally showed increasing trends, and Eh values ​​showed decreasing trend along the flow path, indicating that As was was mobilized via reductive dissolution of Fe oxides. However, in evaporation discharge area, shallow groundwater As was generally lower than those upstream and downstream. In addition to evaporation, siderite and pyrite precipitated controlled groundwater As concentrations. The negative correlations between As concentration and SI may be replenishment of high / low As groundwater for the increase / decrease in As concentrations, resulting from The increase trends in groundwater As concentrations at depth around 15m in the discharge area would result from the increase in the recharge of the underlying groundwater (20m) with high As concentration due to enhanced evaporation in the seasons with high water levels. investigation suggested that monitoring of groundwater As should be routinely carried out to ensure the drinking water safety in the As-affected areas.