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为了对金刚烷胺(AM)和联苯双酯(DDB)抗乙肝病毒(HBV)的作用进行动物实验研究,采用高压注射体内转染法建立HBV复制小鼠模型,对模型小鼠给药3d后处死。用Southern杂交检测小鼠肝脏HBV DNA复制中间体;ELISA检测血清中HBeAg和HBsAg。给予AM和二种药物联合处理后,小鼠体内HBV DNA复制中间体和HBeAg、HBsAg的水平较对照组明显降低,而该两组相比无明显差异。仅给予DDB的小鼠肝脏HBV DNA复制中间体和血清抗原水平与对照组相比无明显差异。因此,AM能够抑制小鼠体内HBV的复制、表达,而连续3d给予DDB对小鼠体内HBV无抑制作用。
In order to study the effects of amantadine (AM) and bifendate (DDB) against hepatitis B virus (HBV) in vivo, HBV replication model was established by high-pressure injection in vivo. After the execution. Southern hybridization was used to detect HBV DNA replication intermediates in mice; ELISA was used to detect HBeAg and HBsAg in serum. Given AM and two drugs combined treatment, the levels of HBV DNA replication intermediates and HBeAg and HBsAg in mice were significantly lower than those in the control group, but there was no significant difference between the two groups. There was no significant difference in liver HBV DNA replication intermediates and serum antigen levels in DDB-only mice compared with the control group. Therefore, AM can inhibit the replication and expression of HBV in mice, while DDB administered continuously for 3d does not inhibit HBV in vivo.