本法以十六烷基三甲基溴化铵为对离子、用所谓皂色谱技术,以硅胶柱,异丙醇:正戊醇:水(65:27:7)为流动相,最佳 pH 为3～4,流量程序为0.8～1ml/min,UV 220 nm 为检测器,可使衍生物获得完全分离。用苦味酸为内标,并藉 Sigma-10数据处理系统,约25分钟便可完成一个样品的分离测定,分别 This method uses cetyltrimethylammonium bromide as the counter ion, so-called soap chromatography technique, and silica gel column, isopropyl alcohol: n-pentanol:water (65:27:7) as the mobile phase, and the optimum pH. For 3-4, the flow rate program is 0.8-1 ml/min, UV 220 nm is the detector, and the derivative can be completely separated. Using picric acid as an internal standard, and using a Sigma-10 data processing system, a sample can be separated and measured in approximately 25 minutes.