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目的探讨并比较丹参提取物764-3和人工合成的超氧化物歧化酶(SOD)对高氧暴露14d新生大鼠高氧肺损伤的早期预防及晚期治疗作用。方法采用SpragueDawley新生大鼠建立高氧肺损伤模型,分别用SOD和丹参提取物在出生后1～7d和7～14d两个不同时间段进行干预,比较高氧对照组、空气对照组、SOD干预组和丹参提取物干预组肺系数、丙二醛(MDA)含量、SOD活性、辐射状肺泡计数(RAC)及肺细胞凋亡指数(AI)。结果除了丹参提取物预防组的AI高于SOD预防组(P<0.05或P<0.01)及SOD治疗组SOD活性高于丹参提取物治疗组外,丹参提取物和SOD对大鼠体重、肺系数、MDA含量及RAC影响的差异,均无统计学意义(P>0.05),且两组的肺系数、MDA含量和AI均低于高氧预防对照组(P<0.01),体重、SOD活性和RAC则高于高氧预防对照组(P<0.05或P<0.01)。结论丹参提取物与SOD一样对新生大鼠高氧肺损伤有一定的预防及治疗作用。
Objective To explore and compare the early prevention and late treatment of hyperoxia - induced lung injury in neonatal rats exposed to hyperoxia for 14 days by Salvia miltiorrhiza extract 764-3 and synthetic superoxide dismutase (SOD). Methods Hyperoxia-induced lung injury model was established in Sprague Dawley rats. SOD and Salvia miltiorrhiza extracts were respectively administered at two different time points of 1 to 7 days and 7 to 14 days after birth. Compared with hyperoxia control group, air control group and SOD intervention The lung coefficient, malondialdehyde (MDA) content, SOD activity, radial alveolar count (RAC) and lung cell apoptosis index (AI) in the intervention group and the Salvia miltiorrhiza extract group were compared. Results In addition to Salvia miltiorrhiza extract prevention group AI higher than the SOD prevention group (P <0.05 or P <0.01) and SOD treatment group SOD activity was higher than Salvia extract treatment group, Salvia extract and SOD body weight, lung coefficient (P <0.05). The lung coefficient, MDA content and AI in the two groups were lower than those in the hyperoxia prevention group (P <0.01), body weight, SOD activity and the difference of MDA content and RAC were not statistically significant RAC was higher than that of hyperoxia prevention group (P <0.05 or P <0.01). Conclusion Salvia miltiorrhiza extract has certain preventive and therapeutic effects on hyperoxia-induced lung injury in neonatal rats, like SOD.