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目的分析南京医科大学第一附属医院2015年临床分离的革兰阴性细菌的分布和耐药情况。方法采用VITEK-2compact自动鉴定仪或API系统进行细菌鉴定,采用纸片扩散法和VITEK-2Compact自动鉴定仪进行药物敏感试验,采用WHONET 5.6软件行药敏分析。结果全年共分离出各类病原菌9137株;其中,革兰阴性细菌5837株,占63.9%。排名前五位的依次是大肠埃希菌、鲍曼不动杆菌、肺炎克雷伯菌、铜绿假单胞菌和嗜麦芽窄食单胞菌。全年共分离到耐碳青霉烯类肠杆菌科细菌289株,占肠杆菌科的8.9%;多重耐药铜绿假单胞菌280株,占铜绿假单胞菌的37.8%;多重耐药鲍曼不动杆菌761株,占鲍曼不动杆菌的65.8%。结论对临床分离细菌进行耐药性监测可掌握细菌药物敏感性的动态变化,有助于指导临床合理应用抗菌药物。
Objective To analyze the distribution and drug resistance of gram-negative bacteria isolated in 2015 from the First Affiliated Hospital of Nanjing Medical University. Methods VITEK-2compact automatic identification system or API system was used for bacterial identification. Drug sensitivity test was carried out by disk diffusion method and VITEK-2 Compact automatic analyzer. WHONET 5.6 software was used for drug susceptibility analysis. Results A total of 9,137 strains of pathogens were isolated in the year. Among them, 5837 were Gram-negative bacteria, accounting for 63.9%. The top five followed by Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia. A total of 289 strains of Enterobacteriaceae resistant to carbapenem were isolated in the year, accounting for 8.9% of Enterobacteriaceae; 280 were multi-resistant Pseudomonas aeruginosa, accounting for 37.8% of Pseudomonas aeruginosa; Acinetobacter baumanni 761, accounting for 65.8% Acinetobacter baumannii. Conclusion Monitoring the drug resistance of clinically isolated bacteria can master the dynamic changes of bacterial drug sensitivity and help guide the rational use of antibacterials in clinic.