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目的:观察自体颈动脉体细胞团块纹状体内移植对慢性帕金森病猴模型行为学评分的影响,为帕金森病的移植治疗提供实验数据。方法:实验于2003-04/2004-04在同济医学院实验动物中心进行,取4只正常恒河猴,采用肌肉注射1-甲基-4苯基-1,2,3,6四氢吡啶(0.1mg/kg,1次/d)制备慢性帕金森病模型。应用帕金森病猴模型修正评分量表进行评价,行为学症状持续稳定1个月后,应用MRI扫描系统进行移植前定位,取一侧的自体颈动脉体,使用Leksell(GM592)脑立体导向系统行纹状体区立体定向移植术,术后按帕金森病猴模型修正评分量表标准进行行为学评分;并采用阿朴吗啡注射诱发旋转的方法观察治疗效果。结果:所有动物均完成了实验,无死亡脱失。移植后2周,动物开始出现帕金森病猴模型修正评分量表评分进展性下降,至移植后4周,行为学评分与移植前比较出现显著性差异(P<0.05);阿朴吗啡注射后动物出现朝向移植侧的旋转行为。结论:自体颈动脉体细胞团块纹状体内移植能够显著促进帕金森病猴的行为康复。
OBJECTIVE: To observe the effect of autologous carotid body mass ciliated striatum transplantation on the behavioral score of chronic Parkinson’s disease monkey model, and to provide experimental data for the transplantation of Parkinson’s disease. METHODS: The experiment was performed in Experimental Animal Center of Tongji Medical College from April 2003 to April 2004. Four normal rhesus monkeys were taken and intramuscular injection of 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (0.1 mg / kg, 1 time / d) to prepare chronic Parkinson’s disease model. The Parkinson’s disease monkey model was used to evaluate the rating scale. After behavioral symptoms persisted for 1 month, the MRI scanning system was used for pre-implantation localization. One side of the autologous carotid body was used and the Leksell (GM592) brain stereotactic system We performed stereotactic grafting in the striatum and scored the behavioral score according to the modified score scale of Parkinson’s disease monkey after operation. The therapeutic effect was observed by apomorphine injection-induced rotation. Results: All animals completed the experiment without loss of life. At 2 weeks after transplantation, the animals started to exhibit progressive decline in the modified model score of Parkinson’s disease monkey model. After 4 weeks of transplantation, the behavioral score showed significant difference compared with that before transplantation (P <0.05); after apomorphine injection Animals appear to rotate toward the graft side. CONCLUSION: Autologous carotid body somatic cell mass transplantation in striatum can significantly promote behavioral rehabilitation in Parkinson’s disease monkeys.