“早熟桃实生苗的发育和成活差,已成为更早熟品种育种工作中的主要障碍。”这主要是由于当果实成熟时胚的发育尚未成熟。为了克服这些困难,已经采用了无菌的胚培养技术。Theobald和Hough谈到了胚培养技术的发展和改进。本文的目的是详尽叙述我们实验室使用的胚培养技术。应当指出,我们使用胚培养方法并不是要作任何努力去挽救由大量杂交产生的那些由于胚乳胚不均衡或遗传上的缺陷等可能在很早就败育了的胚。Theobald和Hough以及我们实验室后来的经验都进一步证实,从花后到果实采收少于72天的胚(子叶达3/4大小),其成苗 “The poor development and survival of precocious peach seedlings has become a major obstacle in the breeding of more precocious varieties.” This is mainly due to the immature embryo development when the fruit matures. In order to overcome these difficulties, sterile embryo culture techniques have been used. Theobald and Hough talked about the development and improvement of embryo culture techniques. The purpose of this article is to describe in detail the embryo culture techniques used in our laboratory. It should be noted that our use of embryo culture methods does not make any effort to save embryos that may have been aborted very early due to imbalances or genetic defects in the endosperm embryo, resulting from a large number of crosses. Theobald and Hough, as well as our lab’s later experience, further confirmed that embryos harvested from post-anthesis to fruit harvest of less than 72 days (cotyledons of 3/4 size)