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目的通过建立人卵巢癌体外耐药模型，研究卵巢癌对顺铂的耐药特征和机制。方法应用浓度递增和短时间作用法。从人卵巢癌细胞A2780中培养对顺铂耐药细胞亚株（A2780／DDP）。采用MTT法检测耐药细胞的耐药指数及对抗癌药物的敏感性。而高效液相色谱仪检测耐药细胞内顺铂含量。以及应用RT－PCR法和免疫组化法检测MDRI和GST－π的表达情况。结果（1）A2780／DDP对DDP的耐药倍数为10．59．并且其倍增时间较A2780细胞延长，对VCR、MTX呈高度耐药状态，对Carp、Pym、CTX、VP16有不同程度的耐药，而对ADM、5－FU、KSM无交叉耐药现象。（2）耐药细胞内顺铂含量明显低于A2780细胞。（3）耐药细胞内GST－π的表达呈强阳性，而MDRI只有在高度耐药细胞呈弱阳性。结论A2780细胞对顺铂的耐药是获得性的，并呈多药耐药特征，其耐药的主要原因是与谷胱甘肽解毒途径有关的酶表达增多有关。GST一π表达过度导致细胞内药物浓度降低有关。
Objective To establish a drug resistance model of human ovarian cancer in vitro to study the characteristics and mechanisms of resistance to cisplatin in ovarian cancer. Methods of increasing concentration and short-term effect. Cisplatin resistant cell subline (A2780 / DDP) was cultured from human ovarian cancer cell A2780. The drug resistance index of drug-resistant cells and the sensitivity to anti-cancer drugs were detected by MTT assay. The HPLC assay of cisplatin-resistant intracellular drug content. RT-PCR and immunohistochemistry were used to detect the expression of MDRI and GST-π. Results (1) A2780 / DDP resistant to DDP multiples of 10.59. The doubling time was longer than that of A2780 cells, and was highly resistant to VCR and MTX. It had different degrees of resistance to Carp, Pym, CTX and VP16, but no cross-resistance to ADM, 5-FU and KSM. (2) The content of cisplatin in resistant cells was significantly lower than that of A2780 cells. (3) The expression of GST-π in drug-resistant cells was strongly positive, while MDRI only showed weakly positive in highly resistant cells. CONCLUSION: A2780 cells are resistant to cisplatin and are multidrug resistant. The main cause of drug resistance is related to the increased expression of enzymes involved in glutathione detoxification pathway. GST-π over-expression led to the reduction of intracellular drug concentration.