论文部分内容阅读
在pH为3的HCl-NaAc缓冲溶液中,硒与吡咯啶二硫代氨基甲酸铵(APDC)可生成稳定的配合物。经40℃水浴15 min,配合物被萃取到Triton X-114表面活性剂相,与水相分开。表面活性剂相可产生强烈的共振瑞利散射信号,最大散射峰位于470 nm,其强度与硒浓度正相关。硒在1～7μg/mL浓度范围与共振散射强度I有良好的线性关系,检出限为0.006 3μg/mL,RSD为2.65%。以所建立的方法测定了富硒螺旋藻片中硒的含量,通过与原子荧光法对比,测定结果无显著性差异。
In HCl-NaAc buffer solution at pH 3, selenium and pyrrolidine dithiocarbamate (APDC) can form stable complexes. The complex was extracted into the Triton X-114 surfactant phase and separated from the aqueous phase after a 40 ° C water bath for 15 min. Surfactant phase can produce strong resonance Rayleigh scattering signal, the maximum scattering peak at 470 nm, the intensity of positive correlation with selenium concentration. The concentration of selenium in the range of 1 ~ 7μg / mL has a good linear relationship with the resonance scattering intensity I, the detection limit is 0.006 3μg / mL, RSD is 2.65%. The content of selenium in Se-enriched spirulina tablets was determined by the established method. There was no significant difference between the two methods by atomic fluorescence spectrometry.