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作者建立了大鼠先天性甲状腺功能减退（甲减）、Ｔ４替代甲减（替代）和正常对照等动物模型，在出生后不同时期抽提脑总ＲＮＡ，经提纯后在３５Ｓ－甲硫氨酸存在的体外兔网织红细胞溶解物系统中表达，翻译蛋白经双向聚丙烯酰胺凝胶电泳分离后作Ｘ线放射自显影，最后用密度图象微机处理。结果表明绝大多数翻译产物在各组之间没有显著的差异，而其中１０种蛋白明显受到甲状腺不同功能状态的影响。在第１４天发育脑中有３种蛋白表达产物甲减鼠中显著高于正常和替代组，而另有２种蛋白却显著低于正常和替代组（Ｐ＜０．０５～ｐ＜０．０１）。而在２１天鼠脑中共有１０种蛋白受甲状腺功能状态的影响，其中３种显著降低而另７种却在正常和替代组中显著升高，变化幅度高于第１４天。但在出生后１～６天之间却无明显改变，２８天与２１天也无明显差异。提示甲状腺激素显然能选择性影响发育中脑的某些特异性基因的表达，并且这种影响作用明显具有一关键时期，这一关键时期处于大鼠出生后的第１４天至第２１天的脑发育分化旺盛期。
The authors established rat models of congenital hypothyroidism (hypothyroidism), T4 hypothyroidism (replacement) and normal control and other animal models, brain RNA extracted at different stages after birth, after purification in the 35S-methionine Existing in vitro rabbit reticulocyte lysate system, the translated protein was separated by two-dimensional polyacrylamide gel electrophoresis and then subjected to X-ray autoradiography, and finally processed with a density image computer. The results showed that most of the translation products in each group there is no significant difference, and of which 10 proteins were significantly affected by different thyroid function status. On the 14th day, there were three protein expression products in hypothyroidism in normal rats and replacement groups, while the other two proteins were significantly lower than those in normal and replacement groups (P <0.05 ~ p <0.05). 01). In the 21 days, a total of 10 proteins in rat brain were affected by thyroid function status. Three of them were significantly reduced while the other seven were significantly increased in the normal and replacement groups, with the amplitude of change being higher than that of the 14th day. However, there was no significant change between 1 and 6 days after birth, and there was no significant difference between 28 and 21 days. It is clear that thyroid hormones apparently selectively affect the expression of some specific genes in the developing brain and this effect obviously has a crucial period, which is between the 14th day and 21st day after birth in rats Strong developmental differentiation.