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目的探讨母乳性黄疸(BMJ)患儿的肠道菌群情况。方法采用鸟枪法宏基因组测序方法,对16例BMJ患儿及16例正常婴儿的肠道菌群进行研究。结果 BMJ组与对照组中样品菌种丰度及菌群种类总的水平上差异无统计学意义(P>0.05);在部分菌种上有差异,双歧杆菌、脆弱拟杆菌及多形拟杆菌在BMJ组中较对照组少(P<0.05);在菌群基因的功能通路上差异也有统计学意义(P≤0.05),尤其是在糖合成和代谢基因功能通路中,BMJ组比对照组表达较少些,差异有统计学意义(P<0.05)。结论 BMJ患儿肠道菌群的种类、丰度与正常同龄婴儿总体上差异不大,在菌种上有部分差异,双歧杆菌、脆弱拟杆菌及多形拟杆菌在BMJ患儿肠道中含量较少,可补充双歧杆菌、拟杆菌制剂使BMJ患儿的肠道菌群平衡。同时,在基因功能通路表达上,BMJ患儿与正常同龄婴儿有显著差别,特别是糖合成和代谢通路,可深入研究。
Objective To investigate the intestinal microflora in children with breast milk jaundice (BMJ). Methods Using shotgun metagenomic sequencing, the intestinal flora of 16 BMJ children and 16 normal infants were studied. Results There was no significant difference in the abundance of samples and the total bacterial species between BMJ group and control group (P> 0.05). There were differences in some species of bacteria, Bifidobacterium, Bacteroides fragilis and polymorphism Bacillus was less in the BMJ group than in the control group (P <0.05). There were also significant differences in the functional pathways among the bacterial gene groups (P≤0.05), especially in the functional pathway of glucose synthesis and metabolism Group less expression, the difference was statistically significant (P <0.05). Conclusion The intestinal flora of BMJ children showed no significant difference in intestinal microflora and intestinal microflora, but there were some differences in the bacterial strains. The contents of Bifidobacterium, Bacteroides fragilis and Bacteroides sb. In the intestine of BMJ children Fewer, can be supplemented with bifidobacteria, Bacteroides preparation BMJ children intestinal flora balance. At the same time, there are significant differences in the expression of gene function pathway between BMJ children and normal infants, especially the sugar synthesis and metabolic pathways, which can be further studied.