论文部分内容阅读
目的:建立同时测定妇炎康片中芍药苷、丹酚酸B及小檗碱的含量测定方法。方法:采用HPLC,PhenomenexLuna C18(4.6 mm×250 mm,5μm),以乙腈-0.05 mol.L-1磷酸二氢钾(含0.1%磷酸)为流动相梯度洗脱,流速1.0 mL.min-1;检测波长230,265,286 nm。结果:芍药苷的线性范围为0.077 08～0.770 8μg(r=0.999 7),平均回收率为98.87%;丹酚酸B的线性范围为0.1139～1.139μg(r=0.999 9),平均回收率为99.31%;小檗碱的线性范围为0.027 8～0.278μg(r=0.999 9),平均回收率为100.65%。不同厂家妇炎康片中芍药苷、丹酚酸B、小檗碱的含量差异较大。结论:该方法简便,专属性、重复性好,可作为该制剂中芍药苷、丹酚酸B及小檗碱的含量测定方法。
Objective: To establish a method for simultaneous determination of paeoniflorin, salvianolic acid B and berberine in Fuyankang tablet. METHODS: HPLC-PhenomenexLuna C18 (4.6 mm × 250 mm, 5 μm) was used to elute acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate with 0.1% phosphoric acid as mobile phase at a flow rate of 1.0 mL · min- ; Detection wavelength 230,265,286 nm. Results: The linear range of paeoniflorin was 0.077 08 ~ 0.770 8μg (r = 0.999 7), the average recovery was 98.87%. The linear range of salvianolic acid B was 0.1139 ~ 1.139μg (r = 0.999 9). The average recovery was 99.31%. The linear range of berberine was 0.027 8 ~ 0.278μg (r = 0.999 9) with the average recovery of 100.65%. Different manufacturers Fu Yan Kang film paeoniflorin, salvianolic acid B, berberine content is quite different. Conclusion: The method is simple, specific, reproducible, and can be used as a method for the determination of paeoniflorin, salvianolic acid B and berberine in the preparation.