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通过利用新一代高通量测序手段——转录组测序(RNA-Seq)技术研究巴西蕉(Musa paradisiaca)叶片在60 mmol/L NaCl人工模拟盐胁迫0、12 h、24 h不同时间点的转录组分析。结果表明:盐胁迫12 h上调和下调的DEGs(differential-expressed genes)分别为2 938个和2 727个;24 h分别有834个和893个。GO富集分析,差异基因主要在细胞过程、代谢过程、刺激响应、结合以及催化活性等。KEGG分析,差异基因主要涉及代谢途径、光合作用、黄酮类生物合成、苯丙素生物合成等。通过GO和KEGG显著性富集分析发现,NaCl胁迫12 h后差异表达的基因数明显多于24 h胁迫后的差异基因表达数。qRT-PCR验证了DEGs的表达趋势与RNA-Sep测序分析结果的一致,证明了RNA-Sep结果的准确性。本研究通过香蕉叶片转录组分析,为研究香蕉耐盐分子机制提供参考。
The transcription of Musa paradisiaca leaves at different time points of 60 mmol / L NaCl artificial salt stress at 0,12 h and 24 h was studied by using a new generation of high-throughput sequencing-RNAi technique. Group analysis. The results showed that DEGs (differential-expressed genes) up-regulated and down-regulated at 12 h were 2 938 and 2 727 respectively, 834 and 893 respectively at 24 h. GO enrichment analysis, differential genes are mainly in cellular processes, metabolic processes, stimulus response, binding and catalytic activity. KEGG analysis, differential genes mainly involved in metabolic pathways, photosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis and so on. Significant enrichment analysis by GO and KEGG showed that the number of differentially expressed genes after 12 h NaCl stress was significantly higher than that after 24 h stress. qRT-PCR verified that the trend of DEGs expression was consistent with the results of RNA-Sep sequencing analysis, demonstrating the accuracy of RNA-Sep results. In this study, banana leaf transcriptome analysis for the study of banana salt-tolerant molecular mechanisms provide a reference.