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食用菌母种被细菌或霉菌等杂菌污染而又要使用时,或者是在进行组织分离时都需要进行纯化分离.纯化分离工作一般都是直接在PDA培养基斜面或平板上进行.这种方法虽然比较简便,但往往不能有效地控制杂菌,特别是霉菌的循环污染.有人通过在培养基中加入一些抗菌素之类的药物加以控制,虽然有一定的抑菌作用,但食用菌的菌丝体也因此而受到了一定程度的药害.笔者在实验中摸索出一种新的纯化分离法——双塞管纯化分离,能较好地解决上述问题.一
Edible fungi are contaminated by bacteria or molds and other bacteria to use, or when the need for tissue separation purification separation.Purification and isolation work is usually directly on the PDA medium slant or plate .This Although the method is relatively simple, but often can not effectively control the bacteria, especially the mold of the circulating pollution Some people by adding some antibiotics and other drugs in the medium to be controlled, although a certain inhibitory effect, but the edible fungus bacteria Therefore, the silk body has been subjected to a certain degree of phytotoxicity.Through experiments, the author has found out a new method of purifying and separating - double-tube purification and separation, which can better solve the above problems.