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目的对2005年湖南省流行性感冒的病原学监测结果进行分析,了解湖南省监测地区流感流行情况,为流感防制提供科学依据。方法采集流感样病例(ILI)的咽拭子标本用狗肾细胞(MDCK)进行病毒分离,采用血凝抑制方法(H_1)进行流感病毒型别鉴定。结果全年共检测2所监测医院ILI咽拭子标本481份,分离到流感病毒57株,阳性分离率为11．85%,经分型鉴定A(H_3N_2)亚型29株,A(H_1N_1)亚型14株,B型7株,7株因HA＜1：8未能分型；疑似流感疫情ILI咽拭子标本144份,分离到流感病毒25株,阳性分离率为17．36%,其中16株为A(H_3N_2)亚型,A(H_1N_1)亚型6株,B型2株,1株未定型。结论2005年湖南省年监测地区全年均有流感流行,流行的优势毒株仍为A(H_3N_2)亚型,但同时有A(H_1N_1)亚型和B型毒株的存在。
Objective To analyze the epidemiological surveillance results of influenza in Hunan Province in 2005 to understand the prevalence of influenza in surveillance areas of Hunan Province and provide a scientific basis for the prevention and control of influenza. Methods Throat swab specimens of influenza-like illness (ILI) were collected for virus isolation using MDCK. The type of influenza virus was identified by hemagglutination inhibition (H 1). Results A total of 482 ILI throat swabs from 2 hospitals under surveillance were detected in the year. 57 strains of influenza virus were isolated. The positive rate of isolation was 11.85%. A (H_3N_2) 14 strains of subtype, 7 strains of B type, 7 strains failed to type due to HA <1: 8, 144 strains of ILI throat swab suspected influenza epidemic, 25 strains of influenza virus were isolated, the positive isolation rate was 17.36% Among them, 16 strains were A (H_3N_2) subtype, 6 strains were A (H_1N_1) subtype, 2 strains were B type and 1 was undefined. Conclusion The annual surveillance area in Hunan Province in 2005 all had influenza pandemic. The predominant strains were still A (H_3N_2) subtype, but there were A (H_1N_1) subtype and B type.