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【目的】反硝化细菌是原位生物修复地下水硝酸盐污染过程中起主要脱氮作用的微生物,通过好氧反硝化细菌去除灌溉农田地下水中的硝酸盐。【方法】将菌株NSA4接种于灌溉农田地下水中,检验其在实际地下水中的脱氮效果。总氮测定采用过硫酸钾氧化紫外分光光度法;氨氮测定采用纳氏试剂分光光度法;硝氮采用酚二磺酸紫外分光光度法测定;亚硝氮测定采用N-(1-奈基)-乙二胺分光光度法。【结果】加菌处理地下水中的NO3--N的去除率要比未加菌处理对NO3--N的去除率高10%~40%,加菌处理对地下水中的TN的去除率要比未加菌处理对TN的去除率高10%左右。【结论】好氧反硝化细菌对于农田灌溉地下水脱氮效率具有显著的改善能力,在未来的地下水处理中具有一定的应用价值。
【Objective】 Denitrifying bacteria is a kind of microorganism that plays a major role in denitrification during in situ bioremediation of groundwater nitrate pollution. Nitrate in irrigation groundwater is removed by aerobic denitrifying bacteria. 【Method】 The strain NSA4 was inoculated into the groundwater of irrigated farmland to test its denitrification effect in the actual groundwater. Determination of total nitrogen using potassium persulfate oxidation UV spectrophotometry; Determination of ammonia nitrogen using Nessler reagent spectrophotometry; Determination of nitrate nitrogen using phenolic disulfonate UV spectrophotometry; Determination of nitrite nitrogen N- (1- Ethylenediamine spectrophotometry. 【Result】 The results showed that the removal rate of NO3 - N in groundwater after adding bacteria was 10% ~ 40% higher than that of NO3 - N without bacteria treatment. The removal rate of TN in groundwater by adding bacteria was higher than that of NO3 - N The removal of bacteria without TN treatment is about 10% higher. 【Conclusion】 Aerobic denitrifying bacteria can significantly improve the nitrogen removal efficiency of farmland irrigation groundwater and has certain value in future groundwater treatment.