作者建立了人血清apoCⅡ单向免疫扩散测定法,系用纯化的人apoCⅡ制备抗血清,抗体浸胶板每孔点样5μl,25℃或37℃扩散48小时测量沉淀环直径。标准曲线工作范围为0.8～4.5mg/dl。最小检测量每孔为40ng。板内及板间变异系数分别为1.4～3.5％及1.3～3.6％。样品回收率为92.38～104.35％。使用本法测定了成都地区67例正常成人和43例高脂血症患者的血清apoCⅡ含量,分别为3.9±1.7和5.9±2.5mg/dl(M±SD)。本法简便、特异、可靠、重复性好,不需特殊仪器。 The authors established a human serum apoC Ⅱ one-way immunodiffusion assay, the purified human apoC Ⅱ antisera were prepared, the antibody dipped plate spot 5μl, 25 ℃ or 37 ℃ for 48 hours to measure the precipitation ring diameter. Standard curve working range of 0.8 ~ 4.5mg / dl. The minimum detection capacity of 40ng per hole. The intraplate and interplate variation coefficients were 1.4-3.5% and 1.3-3.6%, respectively. The recovery rate of samples was 92.38 ~ 104.35%. The method was used to determine the serum apoCⅡlevels in 67 normal adults and 43 hyperlipidemic patients in Chengdu, which were 3.9 ± 1.7 and 5.9 ± 2.5 mg / dl (M ± SD), respectively. This method is simple, specific, reliable, repeatable, without special equipment.