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本文建立了网箱养殖大黄鱼病原菌———副溶血弧菌酶联免疫吸附法 ,也即间接ELISA快速检测方法 .用 0 .5× 1 0 -3 (V/V)甲醛、30℃作用 8h灭活病原菌制成菌苗 .用该菌苗免疫新西兰兔获得特异性抗血清 ,以辣根过氧化物酶标记羊抗兔IgG为标记第二抗体 ,对副溶血弧菌进行间接ELISA快速检测 ,其最低检测极限为 5×1 0 4CFU/cm3 .现埸检测养殖水体中没有副溶血弧菌检出 ,患病大黄鱼肝脏副溶血弧菌的检出率为 70 % ,外观健康大黄鱼肝脏副溶血弧菌的检出率为 2 0 % .现埸检测结果表明 :间接ELISA检测法不仅可以用于患病大黄鱼病原菌的快速检测 ,而且能够检测出无病症带菌大黄鱼 .
This paper established a method of rapid ELISA detection of pathogen of large-yellow croaker cultured in cages --- Vibrio parahaemolyticus by indirect ELISA.Using 0 .5 × 10-3 V / V formaldehyde for 8h at 30 ℃, Inactivated bacterium was used to make bacterin.The New Zealand rabbits were immunized with the bacterin to obtain the specific antiserum.The secondary antibodies were labeled with horseradish peroxidase labeled goat anti-rabbit IgG.And indirect ELISA was used to detect Vibrio parahaemolyticus rapidly, The minimum detection limit of 5 × 104 CFU / cm3. 埸 detection of aquaculture water without Vibrio parahaemolyticus detected, the prevalence of large yellow croaker liver Vibrio parahaemolyticus detection rate of 70%, the appearance of healthy large yellow croaker liver The detection rate of Vibrio parahaemolyticus was 20% .The present results show that indirect ELISA can not only be used for the rapid detection of pathogenic large yellow croaker pathogens, but also can detect the disease-free large yellow croaker.