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目的:研究早期干预对脑损伤后仔鼠脑组织中肝细胞生长因子(HGF)表达的影响。方法:给予孕17天的30只大鼠连续2天腹腔注射脂多糖(LPS)420μg/kg,10只腹腔注射等剂量无菌生理盐水作为对照组。随机选取LPS组仔鼠40只,分为干预组(T)20只与非干预组(NT)20只,生理盐水组(NS)仔鼠20只。仔鼠出生后1日,随机选取各组仔鼠各3只进行脑组织HE染色,并观察脑组织病理改变情况。LPS组和NS组仔鼠出生后均进行早期干预,分别在生后7日、14日、21日、28日的4个时间点行脑组织免疫组织化学染色,观察仔鼠脑组织中HGF的表达及运动功能情况。结果:(1)LPS组仔鼠出生后7日、14日、21日、28日脑组织HGF的表达量较对照组升高(P<0.01);(2)生后7日、14日、21日、28日干预组仔鼠脑组织HGF表达量较非干预组升高(P<0.01)。结论:孕鼠腹腔注射LPS后HGF的表达升高,早期干预可进一步提高其表达量并有效改善脑损伤仔鼠的运动功能。
AIM: To investigate the effect of early intervention on the expression of hepatocyte growth factor (HGF) in brain tissue of neonatal rats with brain injury. Methods: Thirty-seven rats were given intraperitoneal injection of lipopolysaccharide (LPS) 420 μg / kg for 2 days and 10 equal doses of sterile saline as the control group. Forty pups were selected randomly from LPS group, which were divided into intervention group (T) 20 and non-intervention group (NT) 20, saline group (NS) offspring 20. On the 1st day after birth, three offspring of each group were selected randomly to HE staining of brain tissue, and pathological changes of brain tissue were observed. The LPS group and NS group had early intervention after birth, and the brain tissue immunohistochemical staining was performed at 4 time points of 7th, 14th, 21st, 28th day after birth respectively to observe the changes of HGF Expression and motor function. Results: (1) The expression of HGF in LPS group was significantly higher than that in control group on the 7th, 14th, 21st, 28th day after birth (P <0.01). (2) After 7th, 14th, On the 21st and the 28th, the expression of HGF in brain tissue of offspring rats was higher than that of non-intervention group (P <0.01). CONCLUSION: The expression of HGF is increased after intraperitoneal injection of LPS in pregnant rats, and the early intervention can further increase the expression of HGF and improve the motor function of the offspring.