CTL Responses to Regulatory Proteins Tat and Rev in HIV-1 B’/C Virus-Infected Individuals

来源 :Biomedical and Environmental Sciences | 被引量 : 0次 | 上传用户:yhljlb
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B’/C virus-infected ART-na?ve individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-γ ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P<0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-1-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV-1 natural infection and also be useful for the design and testing of candidate vaccines. Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B ’/ C virus-infected ART-naïve individuals. Methods HIV-1-specific CTL responses were analyzed by IFN- γ ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P <0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-1-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV-1 natural infection and also be useful for the design and testing of candidate vaccines.
其他文献
漫长的暑期已经结束,还处在假期的轻松中意犹未尽的你,当回到校园,又开始了紧张的学习,此时,你是否能够将状态尽快调整?面对时隔多日的学习压力,为了防止对新学期的不适应引
经常咀嚼。科研人员发现,咀嚼会刺激脑部主管记忆力的部分,不断地咀嚼可以加快心脏运动,增加头部供应的血液量,从而促进大脑活动,增强人的思维能力。 Chew often. Researche
长这么大,我终于遇到了自己喜欢的女孩子,我很爱她,从我第一眼看到她,我就喜欢上她。她漂亮,开朗,是我喜欢的那种类型的女孩子。  还是从头说起吧。她是我的网友,聊了有一年多了,平时聊天很合的来,可能聊出感觉来了,从今年三月份开始,我开始给她发短信,开始追她,要和她见面。她开始不同意,后来答应我了。五一她们单位组织旅游,她回来时给我发短信,让我去接她。当时已经晚上十点多了(我是研究生,还没有毕业,住宿
用第一人称还是第三人称回忆和讲述自己的故事,结果可能会很不同。心理学家发现,和第一人称相比,用第三人称的方式回忆不好的往事,可以明显减轻不适感。研究者解释说“用第三
期刊
30岁的王女士最近可是伤透了脑筋,她4岁的儿子丁丁开始不听她的话了:早晨起床给他穿衣服他常常会乱发脾气,还总是和她对着干;新衣服没几天就弄出个窟窿来;让他吃饭他 30-yea
心理学上有一个非常著名的实验:研究者将4岁左右的儿童召集在一起,给他们每人发一块棉花糖。并告诉他们: “你们可以马上就吃,但我有事要出去一会儿,谁等我回来再吃,我将给
新时期出现了很多新情况,思想政治工作要随着形势的变化,不断创新工作思路和途径,才能跟上时代发展的需要。 Many new situations have emerged in the new era. Ideologica
通过对邓小平论社会主义生产关系思想的探析,认为:马克思主义政治经济学的分析范式是邓小平论生产关系的基本范式;著名的邓小平社会主义本质论回答了什么是政治经济学的研究对象,并揭示了社会主义生产关系的实质;邓小平的生产关系思想具有独特的表述方式和极其鲜明的辩证思维特征。
为了实现成为一个职业的心理咨询师的梦想,我参加了心理咨询师的职业资格培训。参加培训的人背景不一,几十天的朝夕相处,我们之间形成了一个相互信任、相互支持的团体。后来,
残疾人需要通过文化的参与愉悦身心、陶冶情操,如何对他们的心理倾向和行为予以发现并及时引导,是社区文化建设的一项重要任务。在残疾人社区文化建设背景下,艺术治疗是使社