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1 材料 动物:实验小鼠为雄性昆明种,成年小鼠:3～4个月,生理性肾虚小鼠:12～13个月,由福建医科大学动物中心提供。试剂:S-腺苷酰-~3H-甲基-甲硫氨酸(~3H-SAM)购自NEN公司;刘氏小球菌(Micrococcus Luteus)DNA、鲑鱼鱼精DNA购自Sigma公司;蛋白酶K、PMSF购自Merck公司;DTT、NP-40购自Promega公司。其它试剂均为国产AR级。FJ—2107P液体闪烁计数器由西安262厂生产。高速冷冻离心机为日立20PR—52D。益肾方:由何首乌、仙灵脾、枸杞,川断等组成;健
1 Materials Animals: Experimental mice were male Kunming species, adult mice: 3 to 4 months, physiological kidney deficiency mice: 12 to 13 months, provided by Animal Center of Fujian Medical University. Reagents: S-adenosyl-~3H-methyl-methionine (~3H-SAM) was purchased from NEN; Micrococcus Luteus DNA and salmon carp DNA were purchased from Sigma; proteinase K PMSF was purchased from Merck; DTT and NP-40 were purchased from Promega. Other reagents are domestic AR grades. The FJ-2107P liquid scintillation counter was produced by the Xi’an 262 plant. The high speed refrigerated centrifuge is Hitachi 20PR-52D. Yishen Fang: It is composed of Polygonum multiflorum, epimedium, sputum, and chuanchuan.