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目的从Ｔ细胞中获得更多含ＰＨ结构域编码序列的新基因。方法 采用低严谨核酸杂交技术，根据Ｔ细胞中已知的ＰＨ结构域的分子设计探针，对人Ｔ细胞淋巴瘤ｃＤＮＡ文库进行筛选。结果 在筛选过程中得到了４个新基因，各ｃＤＮＡ长度依次为：１７３３ｂｐ、２３０８ｂｐ、２１３０ｂｐ和１６４７ｂｐ，分别编码含２２９、３７６、２１１ 和 １４３个氨基酸残基的多肽。经生物信息学分析，在基因数据库中未发现与此４种ｃＤＮＡ序列类似的基因。该４个基因均已被ＧｅｎＢａｎｋ收录，登录号分别为：ＡＦ３３４５８８ＡＦ１３４５８９、ＡＦ３３４５９０和 ＡＦ３３４７９２。结论 这些新基因的获得及进一步研究，可能会为深人探讨Ｔ细胞激活机制提供新的线索。
OBJECTIVE: To obtain more new genes containing the coding sequence of PH domain from T cells. Methods A low-stringency nucleic acid hybridization technique was used to screen the human T-cell lymphoma cDNA library based on the molecular design probes of known PH domains in T cells. Results Four novel genes were obtained. The length of each cDNA was 1733bp, 2308bp, 2130bp and 1647bp, encoding 229, 376, 211 and 143 amino acid residues respectively. After bioinformatics analysis, no gene similar to the four cDNA sequences was found in the gene database. The four genes have been deposited in GenBank accession number: AF334588AF134589, AF334590 and AF334792. Conclusion The gain and further study of these new genes may provide new clues for exploring the T cell activation mechanism in depth.