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本文报道了东北地区新病害菜豆菌核病的发生、症状、病原菌的部分生物学特性和苗期抗性的鉴定方法。对病原菌生物学特性的研究结果表明:培养菌3d即可形成菌核雏形,其菌核较黄瓜菌核为小。菌丝的生长温度范围为(1—)8—30℃,以20—21℃最佳,8—25℃时产生菌核。试验了9种不同培养基对菌丝生长速率的影响。以PDA最佳,以在Czapek氏琼脂培养基上产生菌核最多。经与黄瓜、向日葵菌核病菌的交互接种表明:3种菌可交互致病但致病力不同,以向日葵菌最强;寄主以菜豆最为感病。作者在国内首次用限制期接种法在菜豆上进行了苗期抗性鉴定的研究,此种方法可快速有效地诱导菜豆发病,以芹菜段法较好。
This paper reports the occurrence and symptoms of sclerotinia sclerotiorum in northeastern China, as well as some biological characteristics of pathogenic bacteria and identification of seedling stage resistance. The results of the biological characteristics of pathogenic bacteria showed that: the cultured bacteria 3d can form the rupture of sclerotia, and the sclerotium of the bacterial cells is smaller than that of the cucumber sclerotium. Mycelium growth temperature range (1-) 8-30 ℃, the best 20-21 ℃, 8-25 ℃ produce sclerotia. The effects of nine different media on the mycelial growth rate were tested. The PDA was the best to produce the most sclerotia on Czapek’s agar medium. After inoculation with cucumber and sunflower sclerotinia, it showed that the three kinds of bacteria could interact with each other but the pathogenicity was different, and the sunflower was the strongest, while the host was the most susceptible. The authors first conducted the seedling stage identification test on the kidney beans by the restricted period inoculation method. This method can induce the bean disease rapidly and effectively, and the celery section method is better.