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目的 利用裸鼠建立人消化道癌的移植瘤株。利用荷瘤鼠皮下移植瘤组织建立人消化道癌的细胞系。方法 将手术切除的新鲜肿瘤组织剪碎后植入BALB/C裸鼠皮下成瘤。将荷瘤鼠皮下的肿瘤组织取出剪碎后移入培养瓶中,用含15～20％胎牛血清的1640培养基进行培养,建立体外细胞系。结果 建成消化道移植瘤株6株,其中直肠癌2株,结肠癌1株,直肠癌肝转移灶1株,胃癌1株,肝细胞癌1株。建成二例细胞系,分别为直肠癌细胞系(HRC-00)和肝细胞癌细胞系(HHC98)。结论 建成的消化道癌移植瘤株和细胞系均具有人原发癌的结构特点及生物学行为,可作为人消化道癌的体内外研究对象。
Objective To establish a transplanted tumor strain of human digestive tract carcinoma using nude mice. The human gastrointestinal carcinoma cell line was established by subcutaneous transplantation of tumor-bearing mice. Methods Surgically resected fresh tumor tissue was excised and implanted into BALB/C nude mice for subcutaneous tumorigenesis. The subcutaneous tumor tissue of tumor-bearing mice was dissected and transferred into culture flasks, and cultured in 1640 medium containing 15 to 20% fetal bovine serum to establish in vitro cell lines. Results A total of 6 gastrointestinal transplanted tumors were established, including 2 rectal cancers, 1 colon cancer, 1 rectal cancer liver metastasis, 1 gastric cancer, and 1 hepatocellular carcinoma. Two cell lines were established, the rectal cancer cell line (HRC-00) and the hepatocellular carcinoma cell line (HHC98). Conclusion The constructed gastrointestinal cancer xenografts and cell lines all have the structural features and biological behaviors of human primary cancer, which can be used as in vitro and in vivo research subjects for human gastrointestinal cancer.