目的：选用体外培养人多形性胶质母细胞BT325作为对象，研究抗癌药物VM－26的治疗作用机理。方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。结果：实验显示VM－26作用于体外培养人多形性胶质母细胞BT325后可诱导其发生凋亡。结论：VM-26抑制体外培养BT325细胞的生长，是通过阻滞细胞周期的进程，启动细胞自身的调控程序，诱导细胞凋亡，从而达到抗肿瘤的作用。 OBJECTIVE: To study the therapeutic mechanism of anticancer drug VM-26 in vitro by using human glioblastoma BT325 as a subject. Methods: Apoptosis was observed by fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometric spectrophotometry, and DNA gel electrophoresis. Results: The experiment showed that VM-26 could induce apoptosis of human glioblastoma BT325 cells in vitro. Conclusion: VM-26 inhibits the growth of BT325 cells in vitro by inhibiting the progression of the cell cycle, initiating the cell’s own regulatory process, and inducing apoptosis, thereby achieving anti-tumor effects.