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目的:对小儿急性淋巴细胞白血病患者进行HLA基因多态性分型,寻找急性淋巴细胞白血病的易感基因。方法:采用特异性寡核苷酸探针杂交(PCR/SSO)法,对儿童急性淋巴细胞白血病患者和健康对照组进行HLA-A、B、DRB1基因分型。结果:在儿童急性淋巴细胞白血病患者中HLA-A01、A02、HLA-DRB1*01、HLA-DRB1*15基因位点较对照组明显升高(P<0.05)。A11、A33、HLA-DRB1*03基因位点频率较对照组降低(P<0.05)。其中HLA-A01、HLA-DRB1*01、HLA-DRB1*15基因位点相对危险率RR>4,而A11、A33、HLA-DRB1*03基因位点相对危险率RR<1。结论:HLA-A01、A02、A33、HLA-DRB1*01、DRB1*03、DRB1*15与小儿急性淋巴细胞白血病有相关性。其中HLA-A01、HLA-DRB1*01、HLA-DRB1*15对儿童白血病有遗传易感性。A11、A33、HLA-DRB1*03则对青海地区汉族小儿急性淋巴细胞白血病的发生有拮抗作用。
OBJECTIVE: To carry out HLA genotyping in children with acute lymphoblastic leukemia and find susceptible genes for acute lymphoblastic leukemia. Methods: HLA-A, B and DRB1 genotypes were detected in children with acute lymphoblastic leukemia and healthy controls by using specific oligonucleotide probe hybridization (PCR / SSO). Results: The HLA-A01, A02, HLA-DRB1 * 01 and HLA-DRB1 * 15 loci in children with acute lymphoblastic leukemia were significantly higher than those in the control group (P <0.05). The frequency of A11, A33 and HLA-DRB1 * 03 loci was lower than that of the control group (P <0.05). The relative risk of HLA-A01, HLA-DRB1 * 01 and HLA-DRB1 * 15 loci RR> 4, while the relative risk RR of alleles A11, A33 and HLA-DRB1 * 03 was <1. Conclusion: HLA-A01, A02, A33, HLA-DRB1 * 01, DRB1 * 03, DRB1 * 15 are associated with childhood acute lymphoblastic leukemia. Which HLA-A01, HLA-DRB1 * 01, HLA-DRB1 * 15 genetic susceptibility to childhood leukemia. A11, A33 and HLA-DRB1 * 03 antagonized the occurrence of childhood acute lymphoblastic leukemia in Han nationality in Qinghai.