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苹果不同品种叶片再生不定芽能力不同，嘎拉再生能力最强，其次是乔纳金，富士最难再生。用继代培养３ 年以上、当代培养３０ ～５０ ｄ 的试管苗，取顶部第２ ～４ 叶位幼嫩叶片，远轴面向下接触培养基，再生效率高。诱导叶片再生不定芽适宜培养基为ＭＳ＋ ＴＤＺ１．０ ｍｇ·ｌ－ １（ 富士、乔纳金）或ＢＡ５ ．０ ｍｇ·ｌ－ １ ＋ ＮＡＡ０ ．１ ～０．２ ｍｇ·ｌ－ １ ＋ 蔗糖３５ ．０ ｇ·ｌ－ １ ＋ 琼脂６ ．２ ｇ·ｌ－ １（ 嘎拉） 。
Apple different varieties of adventitious bud regeneration ability, Gala regeneration ability strongest, followed by Jonathan, Fuji most difficult to regenerate. With subculture of more than 3 years, the contemporary culture of 30 ~ 50 d of the tube seedlings, take the top of the second to the 4th leaves of young leaves, the far axis contact with the media downwards, high regeneration efficiency. Induction of leaf regeneration of adventitious buds suitable medium for MS + TDZ1.0 mg · l-1 (Fuji, Jonathan) or BA5. 0 mg · l-1 + NAA0. 1 ~ 0.2 mg · l-1 + sucrose 35. 0 g · l-1 + agar 6. 2 g · l -1 (Gala).