论文部分内容阅读
目的：通过对肝硬变大鼠行肝部分切除术基础上，体外活化Kupffer细胞（KC），观察其对肝细胞再生过程的影响。方法：分为肝硬变大鼠部分肝切除术组（C－PH）、正常大鼠部分肝切除术组（N－PH）和假手术组（SO）。皮下注射四氯化碳油溶液加口服乙醇制作大鼠肝硬变模型，切除大鼠肝脏的左叶和中叶。观察时相为大鼠术后6h，行3H－TDR掺合法，3H-亮氨酸的测定，用LPS体外活化KC，观察其对肝再生过程的影响。结果：用小剂量LPS可活化KC，其联合培养的肝细胞DNA合成功能和蛋白质的合成功能均有明显提高，表明活化的KC能促进肝细胞再生。相反。未经活化的KC和肝细胞联合培养（C－PH组），其DNA合成功能和蛋白质合成功能均较单纯的肝细胞培养时的功能有明显降低、能使肝细胞DNA合成达到最大值的LPS浓度为10μg／ml，随着LPS浓度的增高，其肝细胞DNA合成功能反而下降。结论：KC具有促进和抑制肝细胞再生的能力。因此适当地调节KC功能，对促进肝细胞再生具有重要意义。
OBJECTIVE: To investigate the effects of Kupffer cells (KCs) on the regeneration of hepatic cells on the basis of partial hepatectomy in rats with cirrhosis. Methods: The liver partial hepatectomy group (C-PH), the normal partial hepatectomy group (N-PH) and the sham operation group (SO) were divided into two groups. Subcutaneous injection of carbon tetrachloride oil solution plus oral ethanol to produce rat cirrhosis model, excision of the left and middle lobe of rat liver. Observed at 6h after operation, 3H-TDR incorporation method and 3H-leucine assay were performed. The effect of LPS on KC in vitro was observed. Results: KC was activated with low dose of LPS, DNA synthesis and protein synthesis of hepatocytes were significantly increased, indicating that activated KC can promote hepatocyte regeneration. in contrast. Uncoated KC and hepatocyte co-culture (C-PH group), DNA synthesis and protein synthesis were significantly lower than those of pure hepatocyte culture, and LPS Concentration of 10μg / ml, with the increase of LPS concentration, but the function of DNA synthesis of liver cells decreased. Conclusion: KC can promote and inhibit the regeneration of liver cells. Therefore, proper regulation of KC function, to promote liver regeneration is of great significance.