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目的探讨微小核糖核酸(miRNA)-100对人胃癌SGC-7901细胞侵袭、转移能力的影响。方法体外培养人胃癌SGC-7901细胞,将细胞分为空白对照组、阴性对照组和miRNA-100转染组。应用脂质体法,miRNA-100转染组、阴性对照组分别用构建的miRNA模拟物、阴性对照转染SGC-7901细胞。采用逆转录-聚合酶链反应(RTPCR)法检测各组细胞中miRNA-100的表达水平,细胞划痕实验检测细胞的迁移能力,Tranwell实验检测细胞的侵袭能力。结果 RT-PCR结果显示,miRNA-100转染组miRNA-100的表达量明显高于空白对照组和阴性对照组(P均<0.05)。细胞划痕实验显示,与空白对照组及阴性对照组比较,24 h和48 h时miRNA-100组周边细胞迁移至中央划痕区的距离明显增大,差异有统计学意义(P均<0.01)。Transwell实验显示,与空白对照组和阴性对照组相比,转染miRNA-100后,穿越小室的SGC-7901细胞数目明显减少,差异具有统计学意义(P<0.05)。结论转染miRNA-100可使SGC-7901细胞的迁移能力明显降低、细胞的侵袭能力被抑制,上调miRNA-100能抑制人胃癌SGC-7901细胞的侵袭、转移能力。
Objective To investigate the effect of miRNA-100 on invasion and metastasis of human gastric cancer cell line SGC-7901. Methods SGC-7901 cells were cultured in vitro and divided into blank control group, negative control group and miRNA-100 transfected group. The liposome method, miRNA-100 transfected group, negative control group were constructed miRNA mimics, negative control transfected SGC-7901 cells. The expression of miRNA-100 in each group of cells was detected by reverse transcription-polymerase chain reaction (RTPCR). Cell scratch assay was used to detect the migration of cells. Tranwell assay was used to detect the invasion ability of cells. Results The results of RT-PCR showed that the expression of miRNA-100 in miRNA-100 transfected group was significantly higher than that in blank control group and negative control group (all P <0.05). Cell scratch assay showed that compared with the blank control group and the negative control group, the distance from the peripheral miRNA-100 group to the central scratch area at 24 and 48 h was significantly increased (P <0.01, respectively) ). Transwell experiments showed that compared with the blank control group and the negative control group, the number of SGC-7901 cells passing through the cell was significantly decreased after transfection of miRNA-100, the difference was statistically significant (P <0.05). Conclusion Transfection of miRNA-100 can significantly reduce the migration ability of SGC-7901 cells and inhibit the invasion ability of cells. The up-regulation of miRNA-100 can inhibit the invasion and metastasis of SGC-7901 cells.