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目的观察中医温阳健脾法对便秘的治疗作用及机理,以及对实验动物结肠c-kit、SCF蛋白表达的影响。方法运用泻药法复制便秘的大鼠动物模型,采用温阳健脾的中药配方颗粒(生白术/肉苁蓉2∶1)治疗,分成大剂量(9 g/kg)、中剂量(4.5 g/kg)、小剂量(2.25 g/kg),疗程均为4周,并与西药莫沙比利对照。观察大鼠的粪便粒数、重量和形态,采用活性炭悬液推进法,进行肠道运输功能检查,Western-blot方法检测实验大鼠结肠c-kit蛋白、SCF蛋白表达。结果温阳健脾法治疗便秘大鼠有明显疗效,对粪便粒数、重量和形态及肠道运输功能均有改善,与模型组对比有显著差异。温阳健脾法对便秘大鼠c-kit蛋白、SCF蛋白表达的实验结果表明,模型组表达明显低于正常组,差异有统计学意义,治疗后各组c-kit蛋白、SCF蛋白表达有所上升,c-kit蛋白表达治疗组与模型组比,有统计学意义,SCF蛋白表达各治疗组均有所上升,均高于模型组,但无明显统计学意义,可能与样本较少有关。结论温阳健脾法治疗泻药性便秘有明显疗效,泻药性便秘大鼠发生的机理可能与c-kit、SCF蛋白表达下降有关;温阳健脾法可以提高c-kit、SCF蛋白表达。
Objective To observe the therapeutic effect and mechanism of Chinese herbal medicine Warming Yang and Invigorating Spleen on constipation and its effect on the expression of c-kit and SCF in colon of experimental animals. Methods The rat model of constipation was established by laxative method. The rats were treated with Chinese herbal formula granules (Radix Atractylodes / Cistanche Rongli 2: 1) treated with warming Yang and invigorating spleen. The rats were divided into high dose (9 g / kg), medium dose (4.5 g / Dose (2.25 g / kg), the course of treatment was 4 weeks, and compared with Western medicine Mosha Bili. The numbers, weights and morphologies of the feces of the rats were observed. The activated carbon suspension was used to test the intestinal transport function. The expressions of c-kit protein and SCF protein in the colon of rats were detected by Western-blot. Results The method of warming Yang and invigorating the spleen to treat constipation had obvious curative effect on the number, weight and morphology of feces and the function of intestinal transport, which were significantly different from the model group. The results of the experiment of warming Yang and invigorating the spleen on c-kit protein and SCF protein expression in constipation rats showed that the expression of c-kit protein and SCF protein in model group was significantly lower than that in normal group Compared with the model group, the expression of c-kit protein in the treatment group was statistically significant. The expression of SCF protein in each treatment group increased, which was higher than the model group, but not statistically significant, may be related to less samples. Conclusion The method of warming Yang and invigorating the spleen to treat laxative constipation has obvious curative effect. The mechanism of laxative constipation in rats may be related to the decrease of c-kit and SCF protein expression.