首次采用 VIS与酶活性测定的方法 ,研究了溶液中核酸酶 P1与无机氯化铜 ( ) (Cu Cl2 )的直接相互作用。结果发现 :在水溶液中核酸酶 P1活性中心的 Zn( )离子可被外加氯化铜 (Cu Cl2 )中的 Cu( )部分诱导、交换出来 ,而 Cu( )进入酶的活性中心 ,形成相应“Cu( ) - P1”酶衍生物 ,并随着溶液 p H值的变化 ,形成酶的衍生物结构亦随之变化 ,相应地也影响了酶的催化活性。 For the first time, the direct interaction between nuclease P1 and inorganic CuCl 2 (CuCl 2) was studied by means of VIS and enzyme activity assay. The results show that Zn (superscript +) in the active site of nuclease P1 can be partially induced by Cu (superscript 2 +) in CuCl2 and Cu () can enter the active site of the enzyme to form the corresponding “ Cu () - P1 ”enzyme derivatives, and with the p H value of the solution changes, the structure of the enzyme derivatives also changes, correspondingly also affect the catalytic activity of the enzyme.