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目的观察Tenascin(TN)在野百合碱诱导肺动脉高压(肺高压)大鼠右心室组织中的表达变化,探讨其在心室结构重建过程中的作用。方法72只SD大鼠随机分为模型组和对照组,野百合碱一次性皮下注射法复制肺动脉高压模型,右心导管法测定肺动脉压力并计算右室/(左室+室间隔)[RV/(LV+SP)]比值,逆转录聚合酶链反应(RT-PCR)、蛋白免疫印迹(Westernblot)等方法检测TN表达变化。结果用药14d后大鼠出现右心室肥大,模型组大鼠(RV/(LV+SP)(0.282±0.0472),与对照组(0.237±0.154)相比差异有显著性(P<0.05);第21天mPAP升高,模型组大鼠肺动脉压力(26.38±3.86)mmHg明显高于对照组(15.12±1.78)mmHg(P<0.01);TNmRNA在对照组大鼠右心室组织中微量表达,模型组大鼠TNmRNA表达水平于用药7d后显著升高(0.34±0.14),与对照组(0.19±0.07)比较差异有显著性(P<0.01)。模型组大鼠右心室组织中TN-C蛋白表达量明显增加。结论TN在肺动脉高压右心室组织中表达增加,可能在右心室结构重建过程中发挥重要作用。
Objective To observe the changes of Tenascin (TN) in right ventricular tissue induced by monocrotaline-induced pulmonary hypertension (PAH) in rats and its role in the process of ventricular remodeling. Methods Seventy-two SD rats were randomly divided into model group and control group. Hypobaric model was induced by monocrotaline once subcutaneously and pulmonary artery pressure was measured by right heart catheterization. RV / (LV + SP)] ratio, reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of TN. Results Right ventricular hypertrophy occurred on the 14th day after treatment in rats. Compared with the control group (0.237 ± 0.154), RV / (LV + SP) in the model group (0.282 ± 0.0472) was significantly (P <0.05) At 21 days, mPAP increased, and pulmonary hypertension (26.38 ± 3.86) mmHg in the model group was significantly higher than that in the control group (15.12 ± 1.78) mmHg (P <0.01). TNmRNA was slightly expressed in the right ventricular tissue of the control group, Compared with the control group (0.19 ± 0.07), the TNmRNA expression level in the model group was significantly increased (P <0.01) on day 7 after administration (0.34 ± 0.14). The TN-C protein expression in right ventricular tissue The amount of TN in the right ventricular tissue increased significantly, which may play an important role in the reconstruction of right ventricular structure.