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通过生物信息学相关软件分析大豆过敏原Gly m 4蛋白理化性质(Prot Param)、信号肽(Signal P 4.1 Server)、跨膜区(TMHMM Server V 2.0)、B细胞表位(DNAStar)、MHC-Ⅱ类分子的结合能力(Net MHCⅡ2.2)。结果发现:Gly m4蛋白稳定性较好,无信号肽与跨膜区,转角结构丰富;B细胞抗原表位预测表明,Gly m 4蛋白61~64、93~94、122~125、127~130、134~137区域是潜在B细胞抗原表位;MHC-Ⅱ类分子的结合力分析表明Gly m 4蛋白144~153区域及82~96区域是潜在T细胞抗原表位,同时发现HLA-DRB10701、HLA-DRB10101等位基因型人群对Gly m 4蛋白较敏感。Gly m 4蛋白抗原表位分析为大豆过敏原的低过敏原性改造提供参考依据。
Bioinformatics software was used to analyze the genetic polymorphism of Gly m 4 protein in Prot Param, Signal P 4.1 Server, TMHMM Server V 2.0, DNAStar, MHC- Class II molecule binding capacity (Net MHC Ⅱ 2.2). The results showed that the Gly m4 protein had good stability, no signal peptide and transmembrane region, and abundant angled structures. The prediction of B cell epitopes showed that Gly m 4 protein was 61-64, 93-94, 122-125, 127-130 , 134 ~ 137 region is a potential B cell epitopes; MHC-Ⅱ class molecular binding analysis showed Gly m 4 protein 144-153 region and 82-96 region is a potential T cell epitopes, also found HLA-DRB10701, HLA-DRB10101 alleles are more sensitive to Gly m 4 protein. Gly m 4 protein epitope analysis provides a reference for the allergen allergenic transformation of soybean allergens.