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目的:研究多巴胺(DA)对小鼠神经母细胞瘤和大鼠神经胶质瘤的杂交细胞NG108的毒性.方法:用MTT法测定NG108细胞的活性.结果:当DA浓度在100 μmol L~(-1)时对NG108细胞有毒性作用.这时的细胞活性仅为对照的1/4左右.DA受体拮抗剂舒必利和Sch-23390不能阻断DA毒性,表明DA对NG108细胞的毒性作用不是由DA受体介导的.DA(125 μmol L~(-1))的毒性作用能被过氧化氢酶(50 kU L~(-1))、超氧化物歧化酶(50kU L~(-1))和维生素C(200 μmol L~(-1))抑制.它们分别能使NG108细胞的活性由DA单独作用时的25.9±11.0％上升到74.8±4.4％、72.3±4.5％和71.4±2.3％.结论:DA对NG108细胞的毒性作用是由DA氧化代谢产生的有毒产物如过氧化氢引起的.
OBJECTIVE: To study the cytotoxicity of dopamine (DA) on hybridoma NG108 in mouse neuroblastoma and rat glioma. Methods: The activity of NG108 cells was determined by MTT assay. Results: When DA concentration was 100 μmol L~( -1) has a toxic effect on NG108 cells. The cell activity at this time is only about 1/4 of that of the control. The DA receptor antagonists sulpiride and Sch-23390 cannot block DA toxicity, indicating the toxic effect of DA on NG108 cells. The toxicity of DA (125 μmol L-1), which is not mediated by DA receptor, can be catalyzed by catalase (50 kU L-1) and superoxide dismutase (50 kU L-1). -1)) and vitamin C (200 μmol L -1) were inhibited. They could increase the activity of NG108 cells from 25.9±11.0% when DA was applied alone to 74.8±4.4%, 72.3±4.5%, and 71.4, respectively. ±2.3%. Conclusion: The toxic effect of DA on NG108 cells is caused by toxic products such as hydrogen peroxide produced by DA oxidative metabolism.