【摘 要】
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Identifying novel biocontrol agents and developing new strategies are urgent goals in insect pest biocontrol.Ascoviruses are potential competent insect viruses that may be developed into bioinsecticides,but this aim is impeded by their poor oral in-fectiv
【机 构】
：
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Hunan Agr
论文部分内容阅读
Identifying novel biocontrol agents and developing new strategies are urgent goals in insect pest biocontrol.Ascoviruses are potential competent insect viruses that may be developed into bioinsecticides,but this aim is impeded by their poor oral in-fectivity.To improve the per os infectivity of ascovirus,Bacillus thuringiensis kurstaki(Btk) was employed as a helper to damage the midgut of lepidopteran larvae (Helicov-erpa armigera,Mythimna separata,Spodoptera frugiperda,and S.litura) in formulations with Heliothis virescens ascovirus isolates (HvAV-3h and HvAV-3j).Btk and ascovirus mixtures (Btk/HvAV-3h and Btk/HvAV-3j) were fed to insect larvae (3rd instar).With the exception of S.frugiperda larvae,which exhibited low mortality after ingesting Btk,the larvae of the other tested species showed three types of response to feeding on the for-mulas:type Ⅰ,the tested larvae (H.armigera) were killed by Btk infection so quickly that insufficient time and resources remained for ascoviral invasion;type Ⅱ,both Btk and the ascovirus were depleted by their competition,such that neither was successfully released or colonized the tissue;type Ⅲ,Btk was eliminated by the ascovirus,and the ascovirus achieved systemic infection in the tested larvae.The feeding of Btk/ascovirus formulas led to a great reduction in larval diet consumption and resulted in a significant decrease in the emergence rate ofH.armigera,M.separata,and S.litura larvae,which suggested that the formulas exerted marked oral control effects on both the contemporary individuals and the next generation of these tested pest species.
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