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用降落PCR的方法获得了丹参锚蛋白重复序列家族基因(SmARP).生物信息学显示,SmARP所编码蛋白的分子质量为19,064kD,理论等电点为8.4,是一种不稳定而且无信号肽和无跨膜区域的蛋白.该蛋白的二级结构中主要存在α螺旋(39.64%)和无规卷曲(42.01%),其三维结构模型中每个锚蛋白重复序列折叠成两个反向平行的α-螺旋,每两个α-螺旋中间形成一个长环.实时荧光定量PCR结果显示,该基因在丹参的根、茎、叶、花中均有表达.
The SmARP gene of Salvia miltiorrhiza was obtained by the method of landing PCR.The bioinformatics showed that the molecular mass of SmARP protein was 19,064 kD and the theoretical isoelectric point was 8.4, which was an unstable signal peptide without signal And non-transmembrane domain protein.The secondary structure of the protein is mainly α-helix (39.64%) and random coil (42.01%), each of its ankyrin repeats folded into two anti-parallel Of the α-helix, forming a long loop between the two α-helixs.The results of real-time PCR showed that the gene was expressed in roots, stems, leaves and flowers of Salvia miltiorrhiza.